Hexacosanoyl Coenzyme A (ammonium salt) is primarily utilized in biochemical research and metabolic studies. It serves as a key substrate for investigating the enzymatic activity of acyl-CoA synthetases and acyltransferases, which are crucial in lipid metabolism. This compound is also employed in studies focused on understanding the biosynthesis and degradation of very long-chain fatty acids (VLCFAs), which play significant roles in cellular membrane structure and signaling pathways. Additionally, it is used in experiments exploring lipid-related disorders, such as adrenoleukodystrophy, to gain insights into disease mechanisms and potential therapeutic targets. Its application extends to in vitro assays for evaluating enzyme kinetics and substrate specificity in lipid metabolic pathways.