Used as a crosslinking agent in bioconjugation, particularly for coupling proteins and peptides. Its primary application is in the formation of stable amide bonds between primary amines on biomolecules, making it valuable in immunoassay development and labeling of antibodies with enzymes or fluorescent tags. Commonly employed in preparing protein-protein conjugates for diagnostic and research purposes due to its controlled reactivity. Also utilized in immobilizing biomolecules onto surfaces such as biosensors or microplates, where consistent and efficient coupling is required.