Used as an activating agent in peptide synthesis, facilitating the coupling of amino acids by enhancing reaction efficiency and reducing racemization. Commonly employed in the preparation of active esters for bioconjugation, particularly in the development of pharmaceuticals and diagnostic reagents. Its stability and reactivity make it suitable for modifying proteins and linking payloads in antibody-drug conjugates (ADCs). Also applied in solid-phase synthesis due to its compatibility with various protecting groups and mild reaction conditions.