Used in bioconjugation processes to link biotin to proteins, peptides, or other molecules containing primary amines. The N-hydroxysuccinimide ester reacts efficiently with amine groups under mild conditions, forming stable amide bonds. The biotin disulfide component allows for reversible binding due to the reducible disulfide bond, enabling controlled release of the biotinylated molecule in reducing environments. Commonly applied in affinity purification, protein labeling, and detection assays where streptavidin-biotin interaction is utilized. Suitable for creating cleavable biotin tags in proteomics and drug delivery systems.