Used as a bifunctional crosslinking agent in bioconjugation, particularly for coupling peptides or proteins to carrier molecules or surfaces. The N-hydroxysuccinimide (NHS) ester group reacts efficiently with primary amines on biomolecules, forming stable amide bonds, while the palmitoyl chain provides lipophilicity, enhancing membrane association or enabling integration into lipid bilayers. Commonly applied in the development of peptide vaccines, where it links antigenic peptides to immunogenic carriers, improving antigen presentation. Also utilized in immobilizing biomolecules onto biosensor surfaces or functionalizing nanoparticles for targeted drug delivery systems. Its amphiphilic nature allows for controlled orientation and improved stability in biological environments.