Bis-PEG1-PFP ester is widely used in bioconjugation and surface modification applications due to its ability to form stable amide bonds with primary amines. It is particularly valuable in crosslinking biomolecules such as proteins, peptides, and antibodies with other molecules or surfaces, enabling the development of diagnostic probes, targeted drug delivery systems, and biosensors.

Its PFP (pentafluorophenyl) ester groups are highly reactive and offer superior stability compared to NHS esters, allowing conjugation reactions to proceed efficiently under mild conditions and with reduced hydrolysis. This makes it ideal for use in aqueous or mixed solvent systems where maintaining biomolecule integrity is critical.

The short PEG1 (ethylene glycol) spacer provides minimal steric hindrance while improving solubility and flexibility between linked components. It is often used in creating PEGylated compounds, immobilizing ligands on solid supports, and fabricating functionalized nanoparticles.

Additionally, Bis-PEG1-PFP ester serves as a linker in click chemistry strategies and in the synthesis of dendrimers or polymer networks where precise, bifunctional coupling is required.