Used as a chemiluminescent labeling reagent in biochemical and immunoassay applications. It enables detection and quantification of biomolecules by providing strong chemiluminescence upon activation with an oxidant and base after conjugation with amines or other nucleophiles. Commonly employed in enzyme-linked chemiluminescent assays and diagnostic applications due to its high sensitivity and stability. Also utilized in diagnostic kits where signal amplification and low background interference are critical. The compound’s reactivity with primary amines allows for easy conjugation to proteins, antibodies, or peptides, making it suitable for probe development in research and clinical settings.