Anisoyl-NHS is widely used in bioconjugation chemistry for the modification of biomolecules such as proteins, peptides, and antibodies. Its primary application lies in introducing anisoyl groups into amine-containing molecules, enabling the development of labeled biomolecules for use in immunoassays, diagnostic probes, and drug delivery systems. The NHS ester group reacts efficiently with primary amines under mild conditions, forming stable amide bonds, which makes it ideal for coupling reactions in aqueous or organic solvents. It is also employed in the synthesis of photoactivatable or cleavable linkers due to the aromatic and electron-rich nature of the anisoyl moiety, allowing for controlled release or crosslinking in photolabeling experiments. Additionally, it serves as an intermediate in the preparation of enzyme inhibitors and functionalized polymers for biomedical research.