This chemical is primarily used in biochemical research to study cellular processes, particularly those involving actin polymerization. By inhibiting N-WASP, it helps researchers understand the role of this protein in cell motility, membrane trafficking, and cytoskeleton reorganization. It is a valuable tool in cancer research, as it allows scientists to explore how disrupting actin dynamics affects tumor cell invasion and metastasis. Additionally, it is utilized in neuroscience to investigate the mechanisms of synaptic plasticity and neuronal development. Its application extends to drug discovery, where it serves as a reference compound for developing targeted therapies against diseases involving dysregulated actin dynamics.