Dihydrocytochalasin B is widely used in biological research to study cellular processes, particularly those involving the cytoskeleton. It acts as an inhibitor of actin polymerization, which makes it a valuable tool for investigating the role of actin filaments in cell motility, shape, and division. Researchers use it to disrupt the actin network, allowing them to observe the effects on cellular functions such as endocytosis, cytokinesis, and intracellular transport. Additionally, it has applications in cancer research, where it helps in understanding the mechanisms of tumor cell invasion and metastasis, as actin dynamics play a critical role in these processes. Its ability to modulate actin dynamics also makes it useful in studies of muscle contraction and neuronal development.