Mycalolide B is primarily utilized in biological research due to its potent actin-depolymerizing properties. It is extensively used to study the dynamics of actin filaments within cells, providing insights into cellular processes such as motility, division, and structural organization. Researchers employ it to disrupt actin networks, allowing them to observe the effects on cell morphology and function. Additionally, Mycalolide B has shown potential in cancer research, where its ability to inhibit actin polymerization is explored for its effects on tumor cell invasion and metastasis. Its application in these areas makes it a valuable tool for understanding fundamental cellular mechanisms and developing therapeutic strategies.