SMIFH2 is primarily used in biological research to study the role of formin proteins in cellular processes. Formins are involved in the regulation of actin cytoskeleton dynamics, which is crucial for various cellular functions such as cell division, motility, and shape maintenance. By inhibiting formin activity, SMIFH2 helps researchers understand how these proteins contribute to these processes. It is particularly useful in experiments aimed at dissecting the mechanisms of actin polymerization and the formation of cellular structures like filopodia and stress fibers. Additionally, SMIFH2 is employed in studies investigating the impact of formin inhibition on cell migration and cancer cell invasion, providing insights into potential therapeutic targets for diseases where actin dynamics are dysregulated.