N-(L-Phenylalanyl)-2-aminoacridone is primarily utilized in biochemical research as a fluorescent probe. Its application is significant in studying protein interactions and dynamics due to its ability to bind selectively and emit fluorescence upon excitation. This property makes it valuable for fluorescence microscopy and spectroscopy, enabling researchers to visualize and track proteins within cells. Additionally, it is employed in assays to investigate enzyme activity, particularly those involving peptide bonds, as its structure allows for specific interactions with enzymes. The compound's fluorescence characteristics also make it useful in developing diagnostic tools for detecting biomolecules in complex biological samples.