Used in biochemical assays as a fluorescent probe for detecting metal ions and biomolecules due to its strong emission properties. Its sulfonate and carboxyl groups enhance water solubility, making it suitable for use in aqueous biological environments. Commonly applied in fluorescence spectroscopy and imaging techniques to study protein interactions and nucleic acid structures. Also utilized in sensor systems for environmental monitoring, where it helps detect trace amounts of heavy metals in water samples. The compound’s acridinium core contributes to its photostability and high quantum yield, improving sensitivity in diagnostic applications.