Used as a fluorescent dye in biological staining to differentiate between DNA and RNA in cells. Commonly applied in fluorescence microscopy to study cell structures and nucleic acids. Effective in detecting apoptotic cells due to its ability to bind to DNA and emit different colors. Also utilized in flow cytometry for cell cycle analysis. In microbiology, it aids in identifying bacteria and assessing cell viability. Additionally, employed in research for studying chromosome aberrations and nucleic acid interactions.