Used in biochemical research as a fluorescent probe for DNA detection and analysis in techniques such as electrophoresis and microscopy, where it emits fluorescence upon binding to DNA. Its acridine core allows intercalation into DNA strands, disrupting replication and transcription processes, making it useful for studying nucleic acid structure and function. It acts as an inhibitor of DNA-related enzymes, such as topoisomerases, and is employed in studies of antimicrobial and anticancer mechanisms. The acetamido groups enhance solubility and binding specificity, improving its performance in staining and visualization techniques. Also investigated for potential applications in photodynamic therapy due to its light-sensitive properties and ability to generate reactive oxygen species upon irradiation.