Used as a crosslinking agent in bioconjugation, particularly for coupling proteins, peptides, or other biomolecules. Its N-hydroxysuccinimide (NHS) ester groups react efficiently with primary amines (e.g., lysine residues) under mild conditions, forming stable amide bonds. Commonly employed in the preparation of antibody-drug conjugates, immobilization of biomolecules on surfaces (such as biosensors or microarrays), and in the synthesis of cleavable or non-cleavable linkers. The spacer arm provided by the suberic acid chain offers moderate length and hydrophilicity, aiding in maintaining solubility and reducing steric hindrance in conjugated products.