Brilliant Blue G, also known as Coomassie Brilliant Blue G-250, is a widely used dye in biological and biochemical research. It is essential for staining proteins in polyacrylamide gels after electrophoresis, allowing for the visualization, identification, and quantification of proteins, such as in SDS-PAGE and Bradford assays. Additionally, it stains cells and tissues to visualize cellular structures under a microscope, facilitating the study of cell morphology and function. In neuroscience, it is valuable for tracing neural pathways and identifying damaged neurons due to its selective binding to specific cell types. It is also employed in ophthalmic surgeries to stain the inner limiting membrane of the retina, aiding in precise and safe procedures. Its vibrant color and selective staining properties make it a crucial tool in research and medical applications.