o-Acetanisidide is primarily used as an intermediate in the synthesis of various pharmaceuticals and organic compounds. It plays a key role in the production of dyes, particularly azo dyes, which are widely used in the textile industry for coloring fabrics. Additionally, it serves as a precursor in the manufacture of certain agrochemicals, including herbicides and pesticides, contributing to crop protection and agricultural productivity. Its application extends to the field of organic chemistry, where it is utilized in research and development for creating complex molecules with potential therapeutic properties.