IOX 2 is primarily utilized in the field of medicinal chemistry and drug development. It serves as a selective inhibitor of hypoxia-inducible factor prolyl hydroxylase (HIF-PH), making it a valuable tool in research focused on understanding and modulating the hypoxia response pathway. By inhibiting HIF-PH, IOX 2 stabilizes hypoxia-inducible factors (HIFs), which play a critical role in cellular responses to low oxygen levels. This mechanism is particularly relevant in studies related to anemia, as HIF stabilization can stimulate erythropoiesis (red blood cell production). Additionally, IOX 2 is employed in research exploring conditions such as ischemia, cancer, and inflammatory diseases, where hypoxia and HIF signaling pathways are implicated. Its application extends to preclinical studies aimed at developing therapeutic agents targeting oxygen-sensing pathways.