Used in hormone replacement therapy to alleviate symptoms of menopause such as hot flashes, vaginal dryness, and mood changes. Also employed in the treatment of certain hormonal disorders and hypogonadism in women. It helps maintain bone density, reducing the risk of osteoporosis in postmenopausal women. In veterinary medicine, it is used to regulate reproductive cycles in livestock. The acetate ester prolongs the release of estradiol, allowing for less frequent dosing in some formulations.