This compound is primarily utilized in biochemical research, particularly in studies related to cholesterol metabolism and biosynthesis. It serves as an intermediate in the synthesis of various steroidal compounds, aiding in the understanding of enzymatic pathways and mechanisms. Additionally, it is employed in the development of pharmaceuticals targeting cholesterol-related disorders, such as hypercholesterolemia and cardiovascular diseases. Its role in mimicking cholesterol derivatives makes it valuable for investigating lipid dynamics and membrane interactions in cellular systems. Furthermore, it is used in the production of reference standards for analytical methods, ensuring accurate measurement and identification of sterols in biological samples.