Used primarily in medical and biochemical research, this compound serves as a key metabolite of estradiol, aiding in the study of estrogen metabolism and its physiological effects. It is instrumental in developing assays to measure estrogen levels in biological samples, which is crucial for diagnosing and monitoring hormonal disorders. Additionally, it plays a role in pharmacokinetic studies to understand how estradiol is processed and eliminated in the body, contributing to the development of hormone replacement therapies and treatments for conditions like osteoporosis and menopausal symptoms.