This compound is primarily used in peptide synthesis as a protecting group for the amino acid lysine. The tert-butoxycarbonyl (Boc) group shields the alpha-amino group, while the tetradecanoyl group protects the epsilon-amino group, ensuring selective reactions during peptide chain assembly. It is particularly useful in solid-phase peptide synthesis (SPPS), where controlled deprotection and coupling steps are essential. Additionally, it finds applications in the development of bioactive peptides and peptidomimetics, where precise modification of lysine residues is required for enhancing stability, solubility, or biological activity. Its lipophilic tetradecanoyl moiety can also aid in improving membrane permeability of peptide-based drugs.