Used in peptide synthesis as a protected form of histidine, ensuring selective reactions during the construction of complex peptides. It is particularly valuable in solid-phase peptide synthesis (SPPS) where the tert-butoxycarbonyl (Boc) group provides stability under acidic conditions, allowing for precise control over the sequence. The benzyl group offers additional protection for the imidazole side chain, preventing unwanted side reactions. This compound is essential in producing peptides for pharmaceutical research, drug development, and biochemical studies, where high purity and specificity are critical.