This compound is primarily used in peptide synthesis, particularly in solid-phase peptide synthesis (SPPS). It serves as a protected amino acid derivative, where the Fmoc (fluorenylmethyloxycarbonyl) group acts as a temporary protecting group for the amine functionality during the synthesis process. The presence of the 4-pyridyl group in the β-position of the alanine side chain introduces a unique structural feature, which can be exploited to study interactions with proteins or other biomolecules. Its application is significant in the development of peptide-based drugs, where specific side-chain modifications are required to enhance binding affinity, stability, or bioavailability. Additionally, it can be used in the preparation of peptidomimetics or as a building block in the synthesis of complex organic molecules for medicinal chemistry research.