This compound is widely used as a fluorogenic substrate in enzymatic assays, particularly for the detection and quantification of trypsin-like proteases. It is highly sensitive and specific, making it a valuable tool in biochemical research and diagnostics. When cleaved by proteases, it releases a fluorescent product, 7-amino-4-methylcoumarin, which can be easily measured using fluorescence spectroscopy. This property allows researchers to study enzyme kinetics, screen for protease inhibitors, and investigate protease activity in various biological samples. Its application extends to drug discovery, where it aids in identifying potential therapeutic agents targeting proteases. Additionally, it is used in clinical settings to assess protease-related disorders and monitor treatment efficacy.