Used as a substrate in enzymatic assays to study the activity of proteases, particularly trypsin-like enzymes. It helps in measuring enzyme kinetics and inhibition, providing insights into biochemical pathways. Commonly employed in research laboratories for investigating protease functions in various biological processes. Also utilized in diagnostic applications to detect and quantify protease activity in clinical samples. Its specificity makes it valuable in studies related to protein digestion, cellular signaling, and disease mechanisms involving proteolytic enzymes.