Used as a fluorogenic substrate in enzymatic assays, particularly for detecting protease activity. It is commonly employed to measure the activity of glutaryl-proteases or other hydrolases involved in peptide bond cleavage. Upon enzymatic hydrolysis, it releases 4-methoxy-β-naphthylamine, which can be detected fluorometrically, allowing for sensitive and real-time monitoring of enzyme kinetics. This makes it valuable in research settings for studying enzyme inhibition, screening potential drug candidates, and analyzing proteolytic activity in biological samples.