Used in cell signaling research, particularly in studies involving phosphoinositide pathways. Serves as a substrate for enzymes like phosphoinositide 3-kinase (PI3K) and phospholipase C (PLC), helping to investigate intracellular signal transduction mechanisms. Commonly applied in lipid kinase and phosphatase assays to measure enzyme activity and inhibition. Utilized in membrane biology to study lipid-protein interactions and the role of phosphoinositides in membrane trafficking and cellular localization. Often employed in in vitro systems to mimic physiological conditions in neuronal and metabolic signaling studies.