Fluprostenol is primarily used in veterinary medicine to induce estrus and ovulation in livestock, particularly in cattle and horses. It helps synchronize the reproductive cycles of animals, making breeding programs more efficient and predictable. Additionally, it is employed in the management of reproductive disorders in animals, such as luteal cysts, by promoting the regression of the corpus luteum. In research, Fluprostenol is utilized as a potent prostaglandin F2α analog to study its effects on smooth muscle contraction and reproductive physiology. Its ability to mimic natural prostaglandins makes it valuable in both clinical and experimental settings.