This chemical is widely used in biochemical research as a non-hydrolyzable analog of GTP (guanosine triphosphate). It is particularly valuable in studying GTP-binding proteins, such as G-proteins and small GTPases, because it mimics GTP but cannot be broken down by hydrolysis. This allows researchers to investigate the activation and signaling mechanisms of these proteins without the interference of GTPase activity. It is also employed in enzyme assays, cell signaling studies, and structural biology to stabilize protein conformations that are typically transient when bound to GTP. Additionally, it aids in understanding the role of GTP in cellular processes like vesicle trafficking, cytoskeletal dynamics, and signal transduction pathways. Its stability and resistance to enzymatic degradation make it a crucial tool in elucidating the molecular mechanisms of GTP-dependent processes.