Used as a selective inhibitor of nitric oxide synthase (NOS), particularly targeting inducible NOS (iNOS) and neuronal NOS (nNOS). It helps in research settings to modulate nitric oxide levels, which plays a role in inflammation, immune response, and neurotransmission. Commonly applied in studies related to cardiovascular function, neurodegenerative diseases, and septic shock to understand the role of NO in pathophysiological conditions. Also utilized in cellular and animal models to investigate signaling pathways involving nitric oxide.