NF-κB-IN-1 is primarily used in research settings to study the NF-κB signaling pathway, which plays a critical role in inflammation, immune response, and cell survival. By inhibiting this pathway, it helps researchers understand the mechanisms underlying various diseases, including cancer, autoimmune disorders, and chronic inflammatory conditions. It is also employed in drug discovery efforts to develop targeted therapies that modulate NF-κB activity, potentially leading to treatments for conditions where this pathway is dysregulated. Additionally, it serves as a tool in cellular and molecular biology experiments to explore the effects of NF-κB inhibition on gene expression, apoptosis, and cellular responses to stress or infection.