DL-β-Leucine is primarily used in the field of biochemistry and pharmaceutical research. It serves as a building block in the synthesis of various peptides and proteins, aiding in the study of their structures and functions. This compound is also utilized in the development of novel drugs, particularly in the design of enzyme inhibitors and receptor modulators. Additionally, DL-β-Leucine finds application in the production of chiral compounds, where it acts as a precursor or intermediate in asymmetric synthesis. Its role in metabolic studies is significant, as it helps in understanding amino acid metabolism and related biochemical pathways. In industrial biotechnology, DL-β-Leucine is employed in the fermentation process to produce specific amino acids and other bioactive molecules.