4μ8C is primarily utilized in research settings, particularly in the field of neuroscience and pharmacology. It is known for its role as an inhibitor of the integrated stress response (ISR) pathway, specifically targeting the phosphorylation of eukaryotic initiation factor 2 alpha (eIF2α). This mechanism makes it a valuable tool for studying cellular stress responses and their implications in various diseases, including neurodegenerative disorders, cancer, and viral infections. Researchers use 4μ8C to explore how modulating the ISR pathway can influence cell survival, protein synthesis, and disease progression. Its application extends to preclinical studies aimed at developing therapeutic strategies for conditions linked to dysregulated stress responses.