β-Glucuronidase is widely used in biochemical research and clinical diagnostics for its ability to hydrolyze glucuronide conjugates. This enzyme is essential in breaking down complex molecules, making it valuable in drug metabolism studies to analyze metabolites in urine and blood samples. It is also employed in cancer research to detect specific biomarkers, as elevated levels of β-glucuronidase are often associated with certain types of tumors. In the pharmaceutical industry, it aids in the development of prodrugs by releasing active compounds from their glucuronide forms. Additionally, β-glucuronidase is utilized in environmental testing to assess the presence of pollutants by converting glucuronide-bound substances into measurable forms. Its role in these diverse fields highlights its importance in advancing both scientific understanding and practical applications.