β-Glucuronidase from bovine liver is widely used in biochemical research and diagnostic applications due to its ability to hydrolyze glucuronide conjugates. It plays a critical role in the analysis of metabolites in urine and serum, enabling the detection of drugs, hormones, and other compounds that are often excreted as glucuronides. In clinical laboratories, it is employed to deconjugate these metabolites, making them easier to quantify and study. Additionally, this enzyme is utilized in the preparation of samples for chromatography and mass spectrometry, enhancing the accuracy of analytical results. It also finds applications in enzymatic assays to study glucuronidation processes and in the development of therapeutic strategies targeting glucuronide metabolism.