Used as a substrate in enzymatic assays to detect β-glucuronidase activity, which is an important marker in various biological and clinical studies. It helps in identifying bacterial infections, as certain bacteria produce β-glucuronidase. Also employed in histochemistry to localize enzyme activity in tissue samples, aiding in research and diagnostic applications. Its cleavage by the enzyme produces a colored product, making it useful in colorimetric assays for easy detection and quantification.