This compound is widely used as a chromogenic substrate in biochemical and microbiological research. It is particularly valuable in the detection and identification of α-mannosidase enzymes. When the enzyme cleaves the glycosidic bond, it releases an insoluble blue-green precipitate, making it a useful tool for visualizing enzyme activity in assays. This property is leveraged in diagnostic applications, such as detecting bacterial infections, where specific bacteria produce α-mannosidase. Additionally, it is employed in molecular biology for studying glycosidase activity and in the development of enzyme inhibitors. Its ability to provide clear visual results makes it a preferred choice in both research and clinical settings.