Used primarily in biochemical research, this compound serves as a substrate for detecting α-galactosidase enzyme activity. It is particularly valuable in molecular biology and microbiology for identifying and studying bacteria or cells that express this enzyme. When cleaved by α-galactosidase, it produces an insoluble blue product, making it useful for visual assays and colony screening in genetic studies. Its application extends to plant biology for investigating galactose metabolism and enzyme function. Additionally, it supports diagnostic applications for disorders related to α-galactosidase deficiency, such as metabolic abnormalities.