This compound is widely used in biochemical research as a substrate for enzyme assays, particularly for measuring the activity of β-glucuronidase enzymes. It serves as a critical tool in studies related to drug metabolism, as β-glucuronidase is involved in the breakdown of glucuronide conjugates in the body. Additionally, it is employed in diagnostic applications to detect enzyme activity in clinical samples, aiding in the identification of certain diseases or conditions. Its role in research extends to understanding bacterial and mammalian enzyme systems, making it valuable in microbiology and pharmacology studies.