Used primarily in biochemical research, this compound serves as a substrate for enzyme assays, particularly in the study of glucuronidase activity. It helps in detecting and quantifying the presence of β-glucuronidase enzymes, which are important biomarkers in various biological processes. Additionally, it is employed in diagnostic applications to identify bacterial infections, as certain bacteria produce β-glucuronidase. Its role in these assays aids in understanding enzyme kinetics and contributes to the development of diagnostic tools and therapeutic research.