β-Fluoropyruvic acid sodium salt monohydrate is primarily used in biochemical research as a metabolic inhibitor. It plays a significant role in studying enzyme mechanisms, particularly those involving pyruvate metabolism. This compound is utilized to investigate the activity of enzymes like pyruvate kinase and lactate dehydrogenase, helping researchers understand metabolic pathways and their regulation. Additionally, it serves as a tool in cancer research, where it is employed to explore the effects of metabolic disruption on tumor cells. Its ability to inhibit glycolysis makes it valuable in studies focused on energy metabolism and its implications in diseases.