Used as a coupling agent in peptide synthesis, enabling efficient conjugation of carboxylic acid groups to amine groups under mild conditions. Its bifunctional structure supports stepwise assembly of complex molecules, particularly in pharmaceutical and biochemical research. Commonly applied in the development of bioconjugates, sensors, and functional polymers due to its water solubility and stability in aqueous environments. Also utilized in modifying biomolecules like proteins and nucleic acids for diagnostic and therapeutic applications.