6β-Naloxol is primarily used in research and development within the pharmaceutical and medical fields. It serves as a reference material or standard in analytical studies, particularly in the identification and quantification of related compounds. Its application is crucial in the development of opioid antagonists, aiding in the study of their pharmacological properties and mechanisms of action. Additionally, it is utilized in preclinical studies to explore potential therapeutic effects, such as reversing opioid-induced effects or managing opioid dependency. Its role in advancing understanding of opioid receptor interactions makes it valuable in drug discovery and safety assessments.