β-Estradiol 17-acetate is primarily used in hormone replacement therapy (HRT) to address symptoms of menopause, such as hot flashes, vaginal dryness, and osteoporosis. It helps replenish estrogen levels in women experiencing hormonal imbalances. Additionally, it is utilized in the treatment of certain hormonal disorders and as a component in contraceptive formulations. In research, it serves as a reference compound for studying estrogen receptor activity and hormone-related biological processes. Its acetate form enhances stability and bioavailability, making it suitable for various pharmaceutical applications.