Used in biomedical research to measure 17β-hydroxysteroid dehydrogenase (17β-HSD) enzyme activity in biological samples such as cell lysates, tissue extracts, and serum. This kit enables researchers to study steroid hormone metabolism, particularly the interconversion of active and inactive forms of sex hormones like testosterone and estradiol. It is widely applied in endocrinology, reproductive health studies, and cancer research—especially in investigations related to hormone-dependent cancers such as breast and prostate cancer. The assay supports drug development by evaluating the effects of potential inhibitors or activators of 17β-HSD enzymes. Its high sensitivity and specificity make it suitable for monitoring enzyme activity changes under different physiological or pathological conditions.