Used for the quantitative measurement of 7α-hydroxycholesterol (7α-OH cholesterol) levels in bovine serum or plasma samples. This ELISA kit is specifically designed to assess CH7βH (cholesterol 7β-hydroxylase) enzyme activity indirectly by detecting the concentration of its metabolite product. It is widely applied in research related to bile acid synthesis, liver function, and lipid metabolism in cattle. The assay supports studies on metabolic disorders, hepatobiliary diseases, and the impact of drugs or dietary interventions on cholesterol metabolism in bovine models. High sensitivity and specificity make it suitable for use in veterinary research and pharmaceutical development.