Used in biochemical and pharmacological research to study G protein-coupled receptor (GPCR) signaling pathways. Helps in understanding receptor internalization, desensitization, and downstream cellular responses. Commonly applied in assays involving β-arrestin recruitment to assess drug efficacy and bias in GPCR-targeted compounds. Enables real-time monitoring of protein-protein interactions in live cells when tagged with fluorescent or luminescent markers. Supports development of safer therapeutics by identifying compounds with reduced side effects through biased signaling profiles.